Thesis Research
* Senior Project in Biology
Jennifer Sin '96
"The investigation of the function of YCC5, a putative amino acid permease in Saccharomyces cerevisiae."
Abstract
To date, 10 amino acid permease genes have been identified in the yeast, Saccharomyces cerevisiae. The function and specificity of three of the permeases is yet unknown. One of these permeases, YCC5, is located on chromosome III. YCC5 has been found to be highly homologous to a known glutamine permease GNP1 (Michaelis and Garrett, unpublished observations). YCC5 was isolated using restriction enzymes and ligated into the bacterial vector pUC18. A YCC5 mutant was created by inserting the URA3 or HIS3 gene at the BglII restriction site of YCC5. The disrupted gene was transformed into different yeast strains. Transformed strains and the wild-type strains were grown on different amino acid analogs. Differential growth was only seen on the plates containing gamma-hydroxyglutamate. Glutamine uptake assays were also performed.
Jennifer's work is currently submitted as part of a manuscript for publication in the Journal of Bacteriology
The S. cerevisiae YCC5 (YCL025c) gene encodes an amino acid permease, Agp1, which transports asparagine and glutamine.
James L. Schreve, Jennifer K. Sin, and Jinnie M. Garrett